技術優勢

Bontac Bio is a Chinese high-tech enterprise that uses biocatalytic technology to produce coenzyme factors, drug intermediates and natural products on a large scale. We have won the second prize of Shenzhen Science and Technology Progress, and were selected into the top 100 innovative enterprises in Bao'an District. We have built the Coenzyme Engineering Technology Research Center of Guangdong Province and Green Biosynthesis Research Institute.

The R&D center is located in Taohuayuan Innovation and Technology Park, Baoan District, Shenzhen, covering an area of more than 2,000 square meters. The R&D center is mainly responsible for the company's new products and new technology research and development-pilot work, as well as patent application, paper publication, market research, sample testing, project application, technical services, external exchanges and training of young management-level staff. We have completed the mass production of a number of biocatalysis projects on substances such as coenzyme factors (NR , NMN, NAD(H), NADP(H)), creatine phosphate, ginsenoside Rh2, ursodeoxycholic acid, phosphatidylserine, Apigenin, steviol glycoside RD and psicose, etc. In recent years, we have undertaken 5 scientific and technological projects in Shenzhen, received a total of 38 million yuan in vertical funds, applied for more than 150 patents, and owned a library of enzymes of more than 600 kinds.

研發平臺

The R&D center has five major technology platforms respectively for protein expression, enzyme modification, enzyme purification and immobilization, design and optimization of enzyme catalytic reaction condition, and product purification.
 

蛋白表達平臺:


大腸桿菌、酵母菌和鏈黴菌有三種表達宿主。以下是在大腸桿菌中表達蛋白質的發育過程。 1.選擇啟動子、標籤和載體(啟動子、標籤、載體)

2.選擇感受態細胞



 

3.優化搖瓶中的表達溫度和誘導劑用量

4.優化1-20L發酵罐的發酵工藝

 

酶修飾平臺:


核心研發人員具有多年生物催化、結構生物學、定向進化經驗,曾在PNAS、PLoS Pathogens、FEBS J、JMB、JAFC、Biotechnology for Biofuels等知名期刊發表論文。我們集成了(半)理性設計、定向進化、多酶融合等酶工程方法,以改變酶的表達水準、熱穩定性、底物特異性和催化效率。目前,它已應用於輔酶因數、甜菊糖苷RD和psicose等許多專案中。

酶純化固定化平臺:


針對體外酶催化,我們自主研發了多種適合大規模生產的純化樹脂和固定化樹脂。常見的基團包括NTA、IDA、環氧樹脂、氨基和複合基團。還可以根據蛋白質性質和反應條件篩選不同的樹脂基材。 如果底物和產物可以自由進出細胞,並且不易被雜交酶降解,則全細胞催化通常是更好的選擇。

酶催化反應條件設計與優化平臺:


基於成熟的經驗,反應pH值、反應溫度、金屬離子、底物濃度、酶量、反應時間、全細胞/酶保存和應用等反應條件的優化過程可在1-3個月內完成。

 

產品凈化平臺:


針對體外酶催化,我們自主研發了多種適合大規模生產的純化樹脂和固定化樹脂。常見的基團包括NTA、IDA、環氧樹脂、氨基和複合基團。還可以根據蛋白質性質和反應條件篩選不同的樹脂基材。 如果底物和產物可以自由進出細胞,並且不易被雜交酶降解,則全細胞催化通常是更好的選擇。

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